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cytokeratin 14 ck 14  (Proteintech)
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Proteintech cytokeratin 14 ck 14
Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Cytokeratin 14 Ck 14, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytokeratin (ck)14 mouse-monoclonal antibody  (Santa Cruz Biotechnology)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Cytokeratin (Ck)14 Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precellys ceramic beads ck 14s  (Bertin Technologies)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Precellys Ceramic Beads Ck 14s, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck 14 antibody  (Proteintech)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Ck 14 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis tubes soft tissue homogenizing ck 14  (Bertin Technologies)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Lysis Tubes Soft Tissue Homogenizing Ck 14, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck 14 soft tissue homogenizer precellys  (Bertin Technologies)
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Bertin Technologies ck 14 soft tissue homogenizer precellys
Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Ck 14 Soft Tissue Homogenizer Precellys, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck-14 precellys lysine kit  (Bertin Technologies)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Ck 14 Precellys Lysine Kit, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck 14 tubes  (Bertin Technologies)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Ck 14 Tubes, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precellys lysing kit ck 14  (Bertin Technologies)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Precellys Lysing Kit Ck 14, supplied by Bertin Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck 14  (Proteintech)
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Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) <t>CK-14</t> immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.
Ck 14, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) CK-14 immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.

Journal: bioRxiv

Article Title: Insulin-Regulated Actin Dynamics is Disrupted in a Human Keratinocyte Model of Hailey Hailey Disease

doi: 10.64898/2025.12.15.694174

Figure Lengend Snippet: Representative immunofluorescence images of (a) Dsg-1 showing strong peripheral localization in control keratinocytes, but markedly reduced or absent staining in SPCA1 -/- and SPCA1 +/− cells. Magnification, 20x. Scale bar = 20 μm; (b) E-cadherin immunofluorescence showing robust expression at cell junctions in control keratinocytes but near absence in SPCA1 mutants. Magnification, 20x. Scale bar = 20 μm; (c) CK-14 immunofluorescence revealing prominent perinuclear keratin bundles in SPCA1 −/− keratinocytes, with fewer aggregates in SPCA1 +/- cells. Magnification, 40x. Scale bar = 10 μm; and (d) F-actin labeled with fluorescent phalloidin demonstrates abnormal peripheral actin accumulation and cytoplasmic actin-rich structures in SPCA1 mutant cells. Magnification, 40x. Scale bar = 10 μm. (e) Transmission electron micrographs showing intercellular gaps (black arrows), membrane blebs (asterisks) and keratin bundles (red arrowheads) in SPCA1 mutant cells compared to intact junctions (white arrows) in hTERT control. N, nucleus. Scale bar = 2 μm.

Article Snippet: Primary antibodies against ATP2C1, GM130, Cytokeratin 14 (CK-14) (Proteintech #60320) and E-cad were incubated overnight at 4°C or at least 1 h at RT in the blocking buffer at 1:50, 1:300, 1:100 and 1:100 dilution, respectively.

Techniques: Immunofluorescence, Control, Staining, Expressing, Labeling, Mutagenesis, Transmission Assay, Membrane